To correctly identify your pet bird’s sex, the preferred, accurate, trusted method is testing your pet bird’s DNA, a process known as DNA sexing or bird sexing. The DNA sexing procedure is a safe, minimally invasive method. It involves the collection of a blood sample placed on a filter paper and sent to a lab for analysis.
In birds, blood is typically taken from the jugular vein since there is a featherless tract on the neck — the apterylae — which makes the vein easier to spot. Once collected, DNA from the sex chromosomes of a bird is analyzed by DNA sequencing using gel electrophoresis and PCR (Polymerase Chain Reaction) techniques.
Surgical sexing can also be done, but since this procedure is more invasive and requires a general anaesthesia, it is only recommended in certain cases.
Determining the sex of a pet bird can be next to impossible without professional help in some cases.